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goat polyclonal af 266 na  (Bio-Techne corporation)


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    Bio-Techne corporation goat polyclonal af 266 na
    Goat Polyclonal Af 266 Na, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+af+266+na/Human+CXCL10%2FIP-10%2FCRG-2+Antibody/pm38151286-78-23-26
    Average 93 stars, based on 44 article reviews
    goat polyclonal af 266 na - by Bioz Stars, 2026-10
    93/100 stars

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    Immunohistochemistry:

    Article Title: CXCL10 Expression in Human Colorectal Cancer Tissue and its Correlation With Serum Levels of CXCL10.
    Article Snippet: .. For immunohistochemistry, we used primary antibodies mouse monoclonal NCNP24 (WAKO Pure Chemical Industries, Ltd.) to detect Ionized calcium-binding adapter molecule 1 (Iba1) and goat polyclonal AF-266-NA (biotechne) to identify CXCL10. .. The secondary antibody was horseradish peroxidaselabeled anti-mouse or anti-rabbit immunoglobulin antibody (Nichirei, Tokyo, Japan), and positive signals were visualized using 3,3’-diaminobenzidine (Nichirei).



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    FIGURE 2. Immunohistochemical staining for <t>CXCL10</t> and CXCR3 in colonic biopsies from controls, active ulcerative colitis, and active Crohn’s colitis. CXCL10 is located mainly in the epithelial cells, but scattered staining is also seen in the lamina propria. The staining of CXCL10 in epithelial cells is more intense in active ulcerative and active Crohn’s colitis than control. In addition to the cytoplasmatic staining of CXCL10 we see granular staining related to the nucleus. The CXCR3-positive cells are found scattered in the lamina propria and with no difference in diseased vs. healthy control mucosa. Original magnification 20.
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    FIGURE 2. Immunohistochemical staining for CXCL10 and CXCR3 in colonic biopsies from controls, active ulcerative colitis, and active Crohn’s colitis. CXCL10 is located mainly in the epithelial cells, but scattered staining is also seen in the lamina propria. The staining of CXCL10 in epithelial cells is more intense in active ulcerative and active Crohn’s colitis than control. In addition to the cytoplasmatic staining of CXCL10 we see granular staining related to the nucleus. The CXCR3-positive cells are found scattered in the lamina propria and with no difference in diseased vs. healthy control mucosa. Original magnification 20.

    Journal: Inflammatory bowel diseases

    Article Title: Enhanced expression of CXCL10 in inflammatory bowel disease: potential role of mucosal Toll-like receptor 3 stimulation.

    doi: 10.1002/ibd.23034

    Figure Lengend Snippet: FIGURE 2. Immunohistochemical staining for CXCL10 and CXCR3 in colonic biopsies from controls, active ulcerative colitis, and active Crohn’s colitis. CXCL10 is located mainly in the epithelial cells, but scattered staining is also seen in the lamina propria. The staining of CXCL10 in epithelial cells is more intense in active ulcerative and active Crohn’s colitis than control. In addition to the cytoplasmatic staining of CXCL10 we see granular staining related to the nucleus. The CXCR3-positive cells are found scattered in the lamina propria and with no difference in diseased vs. healthy control mucosa. Original magnification 20.

    Article Snippet: These commercially available antibodies were used: CXCL10 goat polyclonal antibody (cat. no. AF-266-NA, R&D Systems, Minneapolis, MN) dilution 1:5, CXCR3 mouse monoclonal antibody (cat. no. ab64714, Abcam, Cambridge, UK) dilution 1:250.

    Techniques: Immunohistochemical staining, Staining, Control

    FIGURE 3. CXCL10 release from HT-29 (A) and SW620 (C) cells stimulated with PRR-ligands corresponding to TLR1-9 and NOD2 (see Table 3) or the cytokines IL-10 and IL-1b for 20 hours. Lowest standard for CXCL10 ELISA; 31.25 pg/mL, indicated by a straight line. *P < 0.05 vs. medium. MTT assays (B,D) show preserved viability of cells irrespective of ligand added. Data given as mean 6 SEM.

    Journal: Inflammatory bowel diseases

    Article Title: Enhanced expression of CXCL10 in inflammatory bowel disease: potential role of mucosal Toll-like receptor 3 stimulation.

    doi: 10.1002/ibd.23034

    Figure Lengend Snippet: FIGURE 3. CXCL10 release from HT-29 (A) and SW620 (C) cells stimulated with PRR-ligands corresponding to TLR1-9 and NOD2 (see Table 3) or the cytokines IL-10 and IL-1b for 20 hours. Lowest standard for CXCL10 ELISA; 31.25 pg/mL, indicated by a straight line. *P < 0.05 vs. medium. MTT assays (B,D) show preserved viability of cells irrespective of ligand added. Data given as mean 6 SEM.

    Article Snippet: These commercially available antibodies were used: CXCL10 goat polyclonal antibody (cat. no. AF-266-NA, R&D Systems, Minneapolis, MN) dilution 1:5, CXCR3 mouse monoclonal antibody (cat. no. ab64714, Abcam, Cambridge, UK) dilution 1:250.

    Techniques: Enzyme-linked Immunosorbent Assay

    FIGURE 4. CXCL10 release in response to poly(I:C) is impaired in HT-29 cells following TLR3 siRNA treatment. HT-29 was left untreated or transfected with TLR3 siRNA, or nonsilencing RNA (ns RNA) for 24 hours, before cells were stimulated with poly(I:C) or medium for 20 hours. CXCL10 content in supernatant was assessed by ELISA. P < 0.05. The results shown as mean 6 SD of triplicates.

    Journal: Inflammatory bowel diseases

    Article Title: Enhanced expression of CXCL10 in inflammatory bowel disease: potential role of mucosal Toll-like receptor 3 stimulation.

    doi: 10.1002/ibd.23034

    Figure Lengend Snippet: FIGURE 4. CXCL10 release in response to poly(I:C) is impaired in HT-29 cells following TLR3 siRNA treatment. HT-29 was left untreated or transfected with TLR3 siRNA, or nonsilencing RNA (ns RNA) for 24 hours, before cells were stimulated with poly(I:C) or medium for 20 hours. CXCL10 content in supernatant was assessed by ELISA. P < 0.05. The results shown as mean 6 SD of triplicates.

    Article Snippet: These commercially available antibodies were used: CXCL10 goat polyclonal antibody (cat. no. AF-266-NA, R&D Systems, Minneapolis, MN) dilution 1:5, CXCR3 mouse monoclonal antibody (cat. no. ab64714, Abcam, Cambridge, UK) dilution 1:250.

    Techniques: Transfection, Enzyme-linked Immunosorbent Assay

    FIGURE 5. CXCL10 release from PBMCs in healthy controls stimu- lated with PRR ligands corresponding to TLR1-9 and NOD2 or the cytokines IL-10 and IL-1b (see Table 3) for 6 hours. Cell supernatant was harvested and assayed for CXCL10 using tge Bio-Plex multi- plexing system. Highest observed value in range at 42,000 pg/mL indicated by a straight line. Nine of nine values for poly(I:C) were above range. Six of nine values for R848 were above range. Data given as mean 6 SEM. The supernatant was diluted 1:10 and ana- lyzed using ELISA CXCL10 for the ligands poly(I:C) and R848 show- ing 5 times higher CXCL10 release in poly(I:C)-stimulated HT-29 compared with R848-stimulated HT-29 (data not shown).

    Journal: Inflammatory bowel diseases

    Article Title: Enhanced expression of CXCL10 in inflammatory bowel disease: potential role of mucosal Toll-like receptor 3 stimulation.

    doi: 10.1002/ibd.23034

    Figure Lengend Snippet: FIGURE 5. CXCL10 release from PBMCs in healthy controls stimu- lated with PRR ligands corresponding to TLR1-9 and NOD2 or the cytokines IL-10 and IL-1b (see Table 3) for 6 hours. Cell supernatant was harvested and assayed for CXCL10 using tge Bio-Plex multi- plexing system. Highest observed value in range at 42,000 pg/mL indicated by a straight line. Nine of nine values for poly(I:C) were above range. Six of nine values for R848 were above range. Data given as mean 6 SEM. The supernatant was diluted 1:10 and ana- lyzed using ELISA CXCL10 for the ligands poly(I:C) and R848 show- ing 5 times higher CXCL10 release in poly(I:C)-stimulated HT-29 compared with R848-stimulated HT-29 (data not shown).

    Article Snippet: These commercially available antibodies were used: CXCL10 goat polyclonal antibody (cat. no. AF-266-NA, R&D Systems, Minneapolis, MN) dilution 1:5, CXCR3 mouse monoclonal antibody (cat. no. ab64714, Abcam, Cambridge, UK) dilution 1:250.

    Techniques: Enzyme-linked Immunosorbent Assay